Mycoplasma Infection of Cell Cultures.pdf

Mycoplasma Infection of Cell Cultures PDF

If one were to design the ideal nuisance for cell cultures, the resultant might well be similar to mycoplasmas. These micro­ organisms are very prevalent in nature, being found in the oral cavity, blood, the mucous membranes of the respiratory and uro­ genital tract and other tissues of both man and animals. They are relatively difficult to detect microbiologically and chemically. Lacking cell walls, they do not routinely produce turbidity in cell cultures and are resistant to antibiotics that act on cell walls. Mycoplasmas grow to high titers in cell cultures. Concen­ 7 8 trations of 10-10 colony forming units per ml of supernatant medium are representative. Additionally, more numbers are attached to cell membranes. Further, mycoplasmas have been shown to mimic in vitro effects of viruses and toxic chemicals. In various cell culture systems, mycoplasmas have been known to cause cell death, decrease or increase cell growth, affect virus tite~s, induce interferon, cause chromosome damage, induce transformation, cyto­ pathic effects, alter phenotypic expression, and significantly alter metabolic pathways and products of cells. The presence of such high concentrations of mycoplasmas in cell cultures constitutes a true in vitro infection. Such infected cell cultures, with a 8 9 total of 10 _10 or more actively metabolizing mycoplasmas, have no place in controlled, standardized cell culture procedures. Numerous reports have been published on mycoplasma assay pro­ cedures, effects of infection, and preventive and elimination measures.

The Cell Culture Test - Mycoplasma Exp THE CELL CULTURE TEST. TESTING WITH MYCOPLASMA EXPERIENCE. After secure transportation from your laboratory under controlled conditions, (see transportation services) the cell culture test is conducted as follows - samples are inoculated into both liquid and solid media, the liquid being subcultured onto solid medium after seven and fourteen days incubation.

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Mycoplasma Infection of Cell Cultures.pdf


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Sofia Voigt

ate mycoplasmas from infected cell cultures: physical, chemi- cal, immunological and chemotherapeutic (= antibiotic) treat- ment (Table 1). Elimination is typically ...

Matteo Müller

How can I eliminate mycoplasma from infected … The quickest and most reliable way to eliminate mycoplasma is to autoclave the contaminated cultures and any bottles of media or sterile reagents that were used with them (Figure 8a).Figure 8a - Autoclaving is the only guaranteed method for mycoplasma elimination. Afterwards, replace them with fresh culture stocks which have been certified as mycoplasma-free.

Noel Schulze

Detection of Mycoplasma in cell cultures | Nature … 22.04.2010 · Mycoplasma is a prokaryotic organism that is a frequent and occult contaminant of cell cultures. This organism can modify many aspects of cell …

Jason Lehmann

Detection of Mycoplasma in cell cultures Mycoplasma contamination of cell cultures is widespread, ranging from 5 to 35% in published reports1. The use of contaminated cells compromises almost all aspects of cell physiology, and consequently the results and conclusions from any experiment2,3. It is essential that all cell stocks and all new cultures entering a laboratory are tested for

Jessica Kohmann

Infected cultures detected by PCR-RFLP were further treated with BM-cyclin (5 μg /mL) and passaged for three times and tested for Mycoplasma infections by PCR-  ... Mycoplasmas are a major cause of cell culture contamination and are especially ... Key words: Adenosine phosphorylase activity; Hidden mycoplasma infection; ...